Proteome profiling of Pseudomonas aeruginosa PAO1 identifies novel responders to copper stress.

ABSTRACT: BACKGROUND:The opportunistic pathogen, Pseudomonas aeruginosa is well known for its environmental and metabolic versatility, yet many of the functions of its gene-products remain to be fully elucidated. This study's objective was to illuminate the potential functions of under-described gene-products during the medically relevant copper-stress condition. RESULTS:We used data-independent acquisition mass spectrometry to quantitate protein expression changes associated with copper stress in P. aeruginosa PAO1. Approximately 2000 non-redundant proteins were quantified, with 78 proteins altering in abundance by +/- 1.5-fold or more when cultured to mid-log growth in the presence of 50 μM copper sulfate. One-third of those differentially expressed proteins have no prior established functional ro

SUBMITTER: Wright BW 

PROVIDER: S-EPMC6444534 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature