Decidual stromal cell-derived PGE₂ regulates macrophage responses to microbial threat.

ABSTRACT:

Problem

Bacterial chorioamnionitis causes adverse pregnancy outcomes, yet host-microbial interactions are not well characterized within gestational membranes. The decidua, the outermost region of the membranes, is a potential point of entry for bacteria ascending from the vagina to cause chorioamnionitis. We sought to determine whether paracrine communication between decidual stromal cells and macrophages shaped immune responses to microbial sensing.

Method of study

Decidual cell-macrophage interactions were modeled in vitro utilizing decidualized, telomerase-immortalized human endometrial stromal cells (dTHESCs) and phorbol ester-differentiated THP-1 macrophage-like cells. The production of inflammatory mediators in response to LPS was monitored by ELISA for both cell types

SUBMITTER: Rogers LM 

PROVIDER: S-EPMC6461368 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature