Estrogen regulation of cardiac cAMP-L-type Ca²⁺ channel pathway modulates sex differences in basal contraction and responses to β₂AR-mediated stress in left ventricular apical myocytes.

ABSTRACT:

Backgrounds/aim

Male and female hearts have many structural and functional differences. Here, we investigated the role of estrogen (E2) in the mechanisms of sex differences in contraction through the cAMP-L-type Ca²⁺channel pathway in adult mice left ventricular (LV) apical myocytes at basal and stress state.

Methods

Isolated LV apical myocytes from male, female (Sham) and ovariectomised mice (OVX) were used to investigate contractility, Ca²⁺ transients and L-type Ca²⁺ channel (LTCC) function. The levels of β₂AR, intracellular cAMP, phosphodiesterase (PDE 3 and PDE 4), RyR2, PLB, SLN, and SERCA2a were compared among the experimental groups.

Results

We found that (1) intracellular cAMP, I_CaL density, contraction an

SUBMITTER: Machuki JO 

PROVIDER: S-EPMC6466778 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature