Estimating transfection efficiency in differentiated and undifferentiated neural cells.

ABSTRACT:

Objective

Delivery of constructs for silencing or over-expressing genes or their modified versions is a crucial step for studying neuronal cell biology. Therefore, efficient transfection is important for the success of these experimental techniques especially in post-mitotic cells like neurons. In this study, we have assessed the transfection rate, using a previously established protocol, in both primary cortical cultures and neuroblastoma cell lines. Transfection efficiencies in these preparations have not been systematically determined before.

Results

Transfection efficiencies obtained herein were (10-12%) for neuroblastoma, (5-12%) for primary astrocytes and (1.3-6%) for primary neurons. We also report on cell-type specific transfection efficiency of neurons and astrocyte

SUBMITTER: Alabdullah AA 

PROVIDER: S-EPMC6466792 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature