The Less the Better: How Suppressed Base Addition Boosts Production of Monoclonal Antibodies With Chinese Hamster Ovary Cells.

ABSTRACT: Biopharmaceutical production processes strive for the optimization of economic efficiency. Among others, the maximization of volumetric productivity is a key criterion. Typical parameters such as partial pressure of CO₂ (pCO₂) and pH are known to influence the performance although reasons are not yet fully elucidated. In this study the effects of pCO₂ and pH shifts on the phenotypic performance were linked to metabolic and energetic changes. Short peak performance of q_mAb (23 pg/cell/day) was achieved by early pCO₂ shifts up to 200 mbar but followed by declining intracellular ATP levels to 2.5 fmol/cell and 80% increase of q_Lac. On the contrary, steadily rising q_mAb could be installed by slight pH down-shifts ensu

SUBMITTER: Becker M 

PROVIDER: S-EPMC6470187 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature