Characterisation of a novel nucleorhabdovirus infecting alfalfa (Medicago sativa).

ABSTRACT:

Background

Nucleorhabdoviruses possess bacilliform particles which contain a single-stranded negative-sense RNA genome. They replicate and mature in the nucleus of infected cells. Together with viruses of three other genera of the family Rhabdoviridae, they are known to infect plants and can be transmitted by arthropod vectors, during vegetative propagation, or by mechanical means. In 2010, an alfalfa (Medicago sativa) plant showing virus-like symptoms was collected from Stadl-Paura, Austria and sent to Julius Kühn Institute for analysis.

Methods

Electron microscopy (EM) of leaf extracts from infected plants revealed the presence of rhabdovirus-like particles and was further used for ultrastructural analyses of infected plant tissue. Partially-purified preparations of rhabdo

SUBMITTER: Gaafar YZA 

PROVIDER: S-EPMC6489223 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature