Increased elasticity of melanoma cells after low-LET proton beam due to actin cytoskeleton rearrangements.

ABSTRACT: Cellular response to non-lethal radiation stress include perturbations in DNA repair, angiogenesis, migration, and adhesion, among others. Low-LET proton beam radiation has been shown to induce somewhat different biological response than photon radiation. For example, we have shown that non-lethal doses of proton beam radiation inhibited migration of cells and that this effect persisted long-term. Here, we have examined cellular elasticity and actin cytoskeleton organization in BLM cutaneous melanoma and Mel270 uveal melanoma cells. Proton beam radiation increased cellular elasticity to a greater extent than X-rays and both types of radiation induced changes in actin cytoskeleton organization. Vimentin level increased in BLM cells after both types of radiation. Our data show that cell elas

SUBMITTER: Jasinska-Konior K 

PROVIDER: S-EPMC6504917 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature