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Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system.

ABSTRACT: The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to including proper controls, our findings suggest that context-specific side effects induced by RU486 should be considered in the experimental design and when interpreting the observed phenotypes.

SUBMITTER: Robles-Murguia M

PROVIDER: S-EPMC6529481 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system.

Robles-Murguia Maricela M Hunt Liam C LC Finkelstein David D Fan Yiping Y Demontis Fabio F

NPJ aging and mechanisms of disease 20190521

The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in <i>Drosophila</i> by adding the drug RU486 to food. The GS system is routinely used in <i>Drosophila</i> aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to incl ...[more]

PMID: 31123597

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