Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system

ABSTRACT: Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system

PROVIDER: PRJNA532894 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8899040_1.fastq.gz	Fastqsanger.gz
	SRR8899040_2.fastq.gz	Fastqsanger.gz
	SRR8899050_1.fastq.gz	Fastqsanger.gz
	SRR8899050_2.fastq.gz	Fastqsanger.gz
	SRR8899041_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system

Project description:The drug-inducible GeneSwitch (GS)-Gal4 transgene expression system is routinely used in Drosophila aging and behavioral studies to avoid the confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. Our findings indicate that side effects induced by RU486 need to be considered when interpreting the observed phenotypes, and proper controls, consisting of ethanol-treated GS-Gal4 flies and RU486-induced expression of mock transgenes, should always be included

2019-06-10 | GSE129815 | GEO

Tissue-specific alteration of gene expression and function by RU486 and the GeneSwitch system.

Project description:The GeneSwitch (GS) is a modified Gal4/UAS system, whereby transgene expression is induced in Drosophila by adding the drug RU486 to food. The GS system is routinely used in Drosophila aging and behavioral studies to avoid confounding effects related to genetic background mutations. Here, we report transcriptional and functional defects that are induced by RU486 in a stock- and tissue-dependent manner, such as defects in flight and mitochondrial gene expression. In addition to including proper controls, our findings suggest that context-specific side effects induced by RU486 should be considered in the experimental design and when interpreting the observed phenotypes.

| S-EPMC6529481 | biostudies-literature

Cell-type-specific modulation of longevity by Forkhead family transcription factors in the Drosophila nervous system

Project description:We previously found that the Drosophila FoxA transcription Fork Head (FKH) is necessary for reduced insulin signalling to extend lifespan. We have now identified that increased expression of FKH in neurons alone is sufficient to extend healthy lifespan. We carried out RNA-sequencing in head tissue on controls (daGS>InRDN,GFP-RNAi without RU-486) and reduced insulin signalling with control RNAi (daGS>InRDN,GFP-RNAi with RU486) and reduced insulin signalling with FKH RNAi (daGS>InRDN,FKH-RNAi with RU486). InR-DN stock used: K1409A.

2021-02-03 | E-MTAB-9017 | biostudies-arrayexpress

Tissue-specific regulation of chromatin insulator function

Project description:Chromatin insulators organize the genome into distinct transcriptional domains and contribute to cell type-specific chromatin organization. However, factors regulating tissue-specific insulator function have not yet been discovered. Here we identify the RNA recognition motif-containing protein, Shep, as a direct interactor of two individual components of the gypsy insulator complex in Drosophila. Mutation of shep improves gypsy-dependent enhancer blocking, indicating a role as a negative regulator of insulator activity. Unlike ubiquitously expressed core gypsy insulator proteins, Shep is highly expressed in the central nervous system (CNS) with lower expression in other tissues. We developed a novel, quantitative tissue-specific barrier assay to demonstrate that Shep functions as a negative regulator of insulator activity in the CNS but not in muscle tissue. Additionally, mutation of shep alters insulator complex nuclear localization in the CNS but not other tissues. Consistent with negative regulatory activity, ChIP-seq analysis of Shep in a CNS-derived cell line indicates substantial genome-wide colocalization with a single gypsy insulator component but limited overlap with intact insulator complexes. Taken together, these data reveal a novel, tissue-specific mode of regulation of a chromatin insulator. ChIP-seq of Shep, Su(Hw), and Mod(mdg4)2.2 in Drosophila BG3 cells along with alternate antibodies

2012-11-30 | E-GEOD-40797 | biostudies-arrayexpress

Tissue-specific non-heritable influence drive uterine immune system variation

Project description:Tissue-specific non-heritable influence drive uterine immune system variation

| PRJNA933605 | ENA

Tissue-specific regulation of chromatin insulator function

2012-11-30 | GSE40797 | GEO

Tissue-specific modifier alleles determine Mertk loss-of-function traits

Project description:Tissue-specific modifier alleles determine Mertk loss-of-function traits

| PRJNA843677 | ENA

Tissue-specific non-heritable influence drive uterine immune system variation (RNA-seq)

Project description:Human twin studies have revealed the combined contribution of heritable and non-heritable (environmental) factors in shaping peripheral blood immune system variability. However, the heterogeneity within tissue immune compartments and the contribution of tissue-specific external factors remain unexplored. The human uterus is a tissue under constant regeneration exposed to distinct environmental factors. To assess uterine immune system variation, we have performed a transcriptomic and epigenetic analysis of uterine and peripheral blood immune cells in monozygotic twins and longitudinal samples in relation to heritable versus non-heritable factors.

2024-04-12 | GSE225041 | GEO

Tissue-specific non-heritable influence drive uterine immune system variation (ATAC-seq)

2024-04-12 | GSE225040 | GEO

Transcription as a function of tissue environment

Project description:Transcription as a function of tissue environment

| PRJNA541514 | ENA