Host and Bacterial Markers that Differ in Children with Cystitis and Pyelonephritis.

ABSTRACT:

Objective

To determine whether treatment for urinary tract infections in children could be individualized using biomarkers for acute pyelonephritis.

Study design

We enrolled 61 children with febrile urinary tract infections, collected blood and urine samples, and performed a renal scan within 2 weeks of diagnosis to identify those with pyelonephritis. Renal scans were interpreted centrally by 2 experts. We measured inflammatory proteins in blood and urine using LUMINEX or an enzyme-linked immunosorbent assay. We evaluated serum RNA expression using RNA sequencing in a subset of children. Finally, for children with Escherichia coli isolated from urine cultures, we performed a polymerase chain reaction for 4 previously identified virulence genes.

Results

Urinary markers

SUBMITTER: Shaikh N 

PROVIDER: S-EPMC6535366 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature