Single cell-derived clonally expanded mesenchymal progenitor cells from somatic cell nuclear transfer-derived pluripotent stem cells ameliorate the endometrial function in the uterus of a murine model with Asherman's syndrome.

ABSTRACT:

Objectives

Because primary mesenchymal progenitor cells (adult-MPCs) have various functions that depend on the tissue origin and donor, de novo MPCs from human pluripotent stem cells (hPSCs) would be required in regenerative medicine. However, the characteristics and function of MPCs derived from reprogrammed hPSCs have not been well studied. Thus, we show that functional MPCs can be successfully established from a single cell-derived clonal expansion following MPC derivation from somatic cell nuclear transfer-derived (SCNT)-hPSCs, and these cells can serve as therapeutic contributors in an animal model of Asherman's syndrome (AS).

Materials and methods

We developed single cell-derived clonal expansion following MPC derivation from SCNT-hPSCs to offer a pure population and a

SUBMITTER: Jun SM 

PROVIDER: S-EPMC6536448 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature