Ontology highlight
ABSTRACT:
SUBMITTER: Xiong K
PROVIDER: S-EPMC6545153 | biostudies-literature | 2019 Jul
REPOSITORIES: biostudies-literature
Biotechnology and bioengineering 20190402 7
Chinese hamster ovary (CHO) cells are widely used for biopharmaceutical protein production. One challenge limiting CHO cell productivity is apoptosis stemming from cellular stress during protein production. Here we applied CRISPR interference (CRISPRi) to downregulate the endogenous expression of apoptotic genes Bak, Bax, and Casp3 in CHO cells. In addition to reduced apoptosis, mitochondrial membrane integrity was improved and the caspase activity was reduced. Moreover, we optimized the CRISPRi ...[more]