Structural and functional characterization of β₂ -glycoprotein I domain 1 in anti-melanoma cell migration.

ABSTRACT: We previously found that circulating β₂ -glycoprotein I inhibits human endothelial cell migration, proliferation, and angiogenesis by diverse mechanisms. In the present study, we investigated the antitumor activities of β₂ -glycoprotein I using structure-function analysis and mapped the critical region within the β₂ -glycoprotein I peptide sequence that mediates anticancer effects. We constructed recombinant cDNA and purified different β₂ -glycoprotein I polypeptide domains using a baculovirus expression system. We found that purified β₂ -glycoprotein I, as well as recombinant β₂ -glycoprotein I full-length (D12345), polypeptide domains I-IV (D1234), and polypeptide domain I (D1) significantly inhibited melanoma cell migrati

SUBMITTER: Leu SJ 

PROVIDER: S-EPMC6549912 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature