Peptide Mapping, In Silico and In Vivo Analysis of Allergenic Sorghum Profilin Peptides.

ABSTRACT:

Background and objectives

Nearly 20-30% of the world's population suffers from allergic rhinitis, among them 15% are progressing to asthma conditions. Sorghum bicolor profilin (Sorb PF), one of the panallergens, was identified, but the allergen specificity is not yet characterized.

Materials and methods

To map the antigenic determinants responsible for IgE binding, the present study is focused on in silico modeling, simulation of Sorb PF and docking of the Sorb PF peptides (PF1-6) against IgG and IgE, followed by in vivo evaluation of the peptides for its allergenicity in mice.

Results

Peptide PF3 and PF4 displayed high docking G-scores (-9.05) against IgE only. The mice sensitized with PF3 peptide showed increased levels of IL5, IL12, TNF-alpha, and GMCSF when

SUBMITTER: Bokka CS 

PROVIDER: S-EPMC6571906 | biostudies-literature | 2019 May

REPOSITORIES: biostudies-literature