New α- and SIN γ-retrovectors for safe transduction and specific transgene expression in pancreatic β cell lines.

ABSTRACT:

Background

Viral vectors are invaluable tools to transfer genes and/or regulatory sequences into differentiated cells such as pancreatic cells. To date, several kinds of viral vectors have been used to transduce different pancreatic cell types, including insulin-producing β cells. However, few studies have used vectors derived from « simple » retroviruses, such as avian α- or mouse γ-retroviruses, despite their high experimental convenience. Moreover, such vectors were never designed to specifically target transgene expression into β cells.

Results

We here describe two novel α- or SIN (Self-Inactivating) γ-retrovectors containing the RIP (Rat Insulin Promoter) as internal promoter. These two retrovectors are easily produced in standard BSL2 conditions, rapidly concentrated i

SUBMITTER: Albagli O 

PROVIDER: S-EPMC6580483 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature