Dp71 depleted HBE cells displayed increased DNA damage and apoptosis induced by H₂O₂.

ABSTRACT: Human bronchial epithelium (HBE)-Dp71 anti-sense(AS)cells with stably transfected Dp71 siRNA plasmids were prepared for further exploration of Dp71 biological traits in cells other than PC12. HBE-Dp71AS cells displayed increased DNA damage induced by H₂O₂. Apoptosis of HBE-Dp71AS cells induced by H₂O₂ was increased via enhancing caspase 3, caspase 8 and caspase 9. HBE-Dp71AS cells also displayed decreased proliferation and clonogenic formation. RAD51 was proved to be a new binding partner of Dp71 by co-immunoprecipitation (Ip) and immunofluorescence. Reduced RAD51 mRNA and protein levels were observed in HBE-Dp71AS cells. Decreased lamin B1, focal adhesion kinase (FAK), phosphorylated focal adhesion kinase (p-FAK) and phosphorylated protein kinas

SUBMITTER: Tan S 

PROVIDER: S-EPMC6580496 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature