Cytotoxic effects of auraptene against a human malignant glioblastoma cell line.

ABSTRACT:

Objective

Glioblastoma multiforme (GBM) is the deadliest type of primary brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of auraptene on U87 GBM cell line.

Materials and methods

The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of auraptene (0-400μg/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide- stained nuclei. Moreover, to determine the Bax, Bcl-2, MCP-1, NF-κB, IL-1β, and p53 genes expression, we used real-time polymerase chain reaction (RT-PCR).

Results

The results revealed that auraptene reduced the viability of U87 ce

SUBMITTER: Afshari AR 

PROVIDER: S-EPMC6612248 | biostudies-literature | 2019 Jul-Aug

REPOSITORIES: biostudies-literature