Ferula gummosa gum exerts cytotoxic effects against human malignant glioblastoma multiforme in vitro.

ABSTRACT:

Background and purpose

Ferula gummosa (F. gummosa), a potent medicinal herb, has been shown to possess anticancer activities in vitro. The present examination evaluated the cytotoxic and apoptogenic impacts of F. gummosa gum on the U87 glioblastoma cells.

Experimental approach

MTT assay to determine the cell viability, flow cytometry by annexin V/FITC-PI to apoptosis evaluation, reactive oxygen species (ROS) assay, and quantitative RT-PCR were performed.

Findings / results

The results revealed that F. gummosa inhibited the growth of U87 cells in a concentration- and time-dependent manner with IC₅₀ values of 115, 82, and 52 μg/mL obtained for 24, 48, and 72 h post-treatment, respectively. It was also identified that ROS lev

SUBMITTER: Afshari AR 

PROVIDER: S-EPMC9661685 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature