Differentiation of adipose-derived stem cells to functional CD105^neg CD73^low melanocyte precursors guided by defined culture condition.

ABSTRACT:

Background

The generation of functional human epidermal melanocytes (HEM) from stem cells provides an unprecedented source for cell-based therapy in vitiligo. Despite the important efforts exerted to obtain melanin-producing cells from stem cells, pre-clinical results still lack the safety and scalability characteristics essential for their translational application.

Methods

Here, we report a rapid and efficient protocol based on defined culture conditions capable of differentiating adult adipose-derived stem cells (ADSC) to scalable amounts of proliferative melanocyte precursors (PreMel) within 30 days. PreMel were characterized in vitro through qPCR, Western blot, flow cytometry, biochemical assays, and in vivo assays in immunocompromised mice (NOD.Cg-Prkdc^scid

SUBMITTER: Zavala G 

PROVIDER: S-EPMC6688240 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature