Hydrogen sulfide lowers hyperhomocysteinemia dependent on cystathionine γ lyase S-sulfhydration in ApoE-knockout atherosclerotic mice.

ABSTRACT:

Background and purpose

Hydrogen sulfide donors can block the cardiovascular injury of hyperhomocysteinemia. H₂ S also lowers serum homocysteine in rats with mild hyperhomocysteinemia, but the pharmacological mechanism is unknown. The present study investigated the mechanism(s) involved.

Experimental approach

ApoE-knockout mice were fed a Paigen diet and L-methionine in drinking water for 16 weeks to create a mouse model of atherosclerosis with hyperhomocysteinemia. H₂ S donors (NaHS and GYY4137) were administered by intraperitoneal injection. We also assayed the H₂ S produced (by methylene blue assay and mito-HS [H₂ S fluorescence probe]), cystathionine γ lyase (CSE) mRNA and protein expression, and CSE sulfhydration and nitrosylat

SUBMITTER: Fan J 

PROVIDER: S-EPMC6692586 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature