Microglia affect α-synuclein cell-to-cell transfer in a mouse model of Parkinson's disease.

ABSTRACT:

Background

Cell-to-cell propagation of α-synuclein (α-syn) aggregates is thought to contribute to the pathogenesis of Parkinson's disease (PD) and underlie the spread of α-syn neuropathology. Increased pro-inflammatory cytokine levels and activated microglia are present in PD and activated microglia can promote α-syn aggregation. However, it is unclear how microglia influence α-syn cell-to-cell transfer.

Methods

We developed a clinically relevant mouse model to monitor α-syn prion-like propagation between cells; we transplanted wild-type mouse embryonic midbrain neurons into a mouse striatum overexpressing human α-syn (huα-syn) following adeno-associated viral injection into the substantia nigra. In this system, we depleted or activated microglial cells and determined the ef

SUBMITTER: George S 

PROVIDER: S-EPMC6697982 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature