Dataset Information
Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine.
Ontology highlight
ABSTRACT: Purpose
To characterize the global profile of circular RNAs (circRNAs) and their differential expression levels in homocysteine (Hcy)-treated ARPE-19 cells, a line of human retinal pigment epithelial (RPE) cells.Materials and methods
We treated ARPE-19 cells with and without Hcy to investigate the influence of Hcy on circRNA expression levels using dedicated human circRNA microarrays.Results
A total of 12,233 circRNAs were identified out of them 54 were differentially expressed (17 were down-regulated, and 37 were up-regulated) with a fold change >2.0 (p < 0.05) in Hcy-treated versus untreated cells.Conclusions
To our knowledge, this is the first report profiling circRNAs in human RPE cells post-Hcy treatment mimicking hyperhomocysteinemic (HHcy) conditions that negatively affect retinal biology and vision. These findings are of potential clinical significance as they will help understand Hcy metabolism and HHcy-mediated diseases and identify potential diagnostic and therapeutic targets for eye diseases that are caused by elevated Hcy concentrations.
SUBMITTER: Singh M
PROVIDER: S-EPMC6709669 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Publications
Expression Analysis of the Circular RNA Molecules in the Human Retinal Cells Treated with Homocysteine.
Current eye research 20181205 3
<h4>Purpose</h4>To characterize the global profile of circular RNAs (circRNAs) and their differential expression levels in homocysteine (Hcy)-treated ARPE-19 cells, a line of human retinal pigment epithelial (RPE) cells.<h4>Materials and methods</h4>We treated ARPE-19 cells with and without Hcy to investigate the influence of Hcy on circRNA expression levels using dedicated human circRNA microarrays.<h4>Results</h4>A total of 12,233 circRNAs were identified out of them 54 were differentially exp ...[more]