Sequential analysis of myocardial gene expression with phenotypic change: Use of cross-platform concordance to strengthen biologic relevance.

ABSTRACT:

Objectives

To investigate the biologic relevance of cross-platform concordant changes in gene expression in intact human failing/hypertrophied ventricular myocardium undergoing reverse remodeling.

Background

Information is lacking on genes and networks involved in remodeled human LVs, and in the associated investigative best practices.

Methods

We measured mRNA expression in ventricular septal endomyocardial biopsies from 47 idiopathic dilated cardiomyopathy patients, at baseline and after 3-12 months of β-blocker treatment to effect left ventricular (LV) reverse remodeling as measured by ejection fraction (LVEF). Cross-platform gene expression change concordance was investigated in reverse remodeling Responders (R) and Nonresponders (NR) using 3 platforms (RT-qPCR, mi

SUBMITTER: Toni LS 

PROVIDER: S-EPMC6716635 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature