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New perspectives for viability studies with high-content analysis Raman spectroscopy (HCA-RS).

ABSTRACT: Raman spectroscopy has been widely used in clinical and molecular biological studies, providing high chemical specificity without the necessity of labels and with little-to-no sample preparation. However, currently performed Raman-based studies of eukaryotic cells are still very laborious and time-consuming, resulting in a low number of sampled cells and questionable statistical validations. Furthermore, the approach requires a trained specialist to perform and analyze the experiments, rendering the method less attractive for most laboratories. In this work, we present a new high-content analysis Raman spectroscopy (HCA-RS) platform that overcomes the current challenges of conventional Raman spectroscopy implementations. HCA-RS allows sampling of a large number of cells under different physiological conditions without any user interaction. The performance of the approach is successfully demonstrated by the development of a Raman-based cell viability assay, i.e., the effect of doxorubicin concentration on monocytic THP-1 cells. A statistical model, principal component analysis combined with support vector machine (PCA-SVM), was found to successfully predict the percentage of viable cells in a mixed population and is in good agreement to results obtained by a standard cell viability assay. This study demonstrates the potential of Raman spectroscopy as a standard high-throughput tool for clinical and biological applications.

SUBMITTER: Mondol AS

PROVIDER: S-EPMC6718400 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature

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New perspectives for viability studies with high-content analysis Raman spectroscopy (HCA-RS).

Mondol Abdullah Saif AS Töpfer Natalie N Rüger Jan J Neugebauer Ute U Popp Jürgen J Schie Iwan W IW

Scientific reports 20190902 1

Raman spectroscopy has been widely used in clinical and molecular biological studies, providing high chemical specificity without the necessity of labels and with little-to-no sample preparation. However, currently performed Raman-based studies of eukaryotic cells are still very laborious and time-consuming, resulting in a low number of sampled cells and questionable statistical validations. Furthermore, the approach requires a trained specialist to perform and analyze the experiments, rendering ...[more]

PMID: 31477762

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New perspectives for viability studies with high-content analysis Raman spectroscopy (HCA-RS).

Publications

New perspectives for viability studies with high-content analysis Raman spectroscopy (HCA-RS).

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