Highly efficacious antiviral protection of plants by small interfering RNAs identified in vitro.

ABSTRACT: In response to a viral infection, the plant's RNA silencing machinery processes viral RNAs into a huge number of small interfering RNAs (siRNAs). However, a very few of these siRNAs actually interfere with viral replication. A reliable approach to identify these immunologically effective siRNAs (esiRNAs) and to define the characteristics underlying their activity has not been available so far. Here, we develop a novel screening approach that enables a rapid functional identification of antiviral esiRNAs. Tests on the efficacy of such identified esiRNAs of a model virus achieved a virtual full protection of plants against a massive subsequent infection in transient applications. We find that the functionality of esiRNAs depends crucially on two properties: the binding affinity to Argonaute

SUBMITTER: Gago-Zachert S 

PROVIDER: S-EPMC6755098 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature