Metabolic Regulation of Organic Acid Biosynthesis in Actinobacillus succinogenes.

ABSTRACT: Actinobacillus succinogenes is one of the most promising strains for succinic acid production; however, the lack of efficient genetic tools for strain modification development hinders its further application. In this study, a markerless knockout method for A. succinogenes using in-frame deletion was first developed. The resulting ΔpflA (encode pyruvate formate lyase 1-activating protein) strain displayed distinctive organic acid synthesis capacity under different cultivation modes. Additional acetate accumulation was observed in the ΔpflA strain relative to that of the wild type under aerobic conditions, indicating that acetate biosynthetic pathway was activated. Importantly, pyruvate was completely converted to lactate under anaerobic fermentation. The transcription analysis and enzyme as

SUBMITTER: Zhang W 

PROVIDER: S-EPMC6759810 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature