Detection and molecular characterization of urinary tract HIV-1 populations.

ABSTRACT:

Background

Identification of all possible HIV reservoirs is an important aspect in HIV eradication efforts. The urinary tract has however not been well studied as a potential HIV reservoir. In this pilot study we molecularly characterized HIV-1 viruses in urine and plasma samples to investigate HIV-1 replication, compartmentalization and persistence in the urinary tract.

Methods

Prospectively collected urine and blood samples collected over 12-36 months from 20 HIV-1 infected individuals were analysed including sampling points from prior to and after ART initiation. HIV-1 pol gene RNA and DNA from urine supernatant and urine pellets respectively were analysed and compared to plasma RNA viruses from the same individual.

Results

HIV-1 nucleic acid was detected in urine

SUBMITTER: Mzingwane ML 

PROVIDER: S-EPMC6760049 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature