Genetic compensation by epob in pronephros development in epoa mutant zebrafish.

ABSTRACT: Zebrafish erythropoietin a (epoa) is a well characterized regulator of red blood cell formation. Recent morpholino mediated knockdown data have also identified epoa being essential for physiological pronephros development in zebrafish, which is driven by blocking apoptosis in developing kidneys. Yet, zebrafish mutants for epoa have not been described so far. In order to compare a transient knockdown vs. permanent knockout for epoa in zebrafish on pronephros development, we used CRISPR/Cas9 technology to generate epoa knockout zebrafish mutants and we performed structural and functional studies on pronephros development. In contrast to epoa morphants, epoa^-/- zebrafish mutants showed normal pronephros structure; however, a previously unch

SUBMITTER: She J 

PROVIDER: S-EPMC6773226 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature