Ontology highlight
ABSTRACT:
SUBMITTER: Vahrenkamp JM
PROVIDER: S-EPMC6836741 | biostudies-literature | 2019 Nov
REPOSITORIES: biostudies-literature
Vahrenkamp Jeffery M JM Szczotka Kathryn K Dodson Mark K MK Jarboe Elke A EA Soisson Andrew P AP Gertz Jason J
Genome research 20191024 11
The majority of clinical cancer specimens are preserved as formalin-fixed paraffin-embedded (FFPE) samples. For clinical molecular tests to have wide-reaching impact, they must be applicable to FFPE material. Accurate quantitative measurements of RNA derived from FFPE specimens is challenging because of low yields and high amounts of degradation. Here, we present FFPEcap-seq, a method specifically designed for sequencing capped 5' ends of RNA derived from FFPE samples. FFPEcap-seq combines enzym ...[more]