FACT-seq: profiling histone modifications in formalin-fixed paraffin-embedded samples with low cell numbers.

ABSTRACT: The majority of biopsies in both basic research and translational cancer studies are preserved in the format of archived formalin-fixed paraffin-embedded (FFPE) samples. Profiling histone modifications in archived FFPE tissues is critically important to understand gene regulation in human disease. The required input for current genome-wide histone modification profiling studies from FFPE samples is either 10-20 tissue sections or whole tissue blocks, which prevents better resolved analyses. But it is desirable to consume a minimal amount of FFPE tissue sections in the analysis as clinical tissues of interest are limited. Here, we present FFPE tissue with antibody-guided chromatin tagmentation with sequencing (FACT-seq), the first highly sensitive method to efficiently profile histone modif

SUBMITTER: Zhao L 

PROVIDER: S-EPMC8643707 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature