Genetic Lineage Tracing of Sca-1⁺ Cells Reveals Endothelial but Not Myogenic Contribution to the Murine Heart.

ABSTRACT:

Background

The adult mammalian heart displays a cardiomyocyte turnover rate of ≈1%/y throughout postnatal life and after injuries such as myocardial infarction (MI), but the question of which cell types drive this low level of new cardiomyocyte formation remains contentious. Cardiac-resident stem cells marked by stem cell antigen-1 (Sca-1, gene name Ly6a) have been proposed as an important source of cardiomyocyte renewal. However, the in vivo contribution of endogenous Sca-1⁺ cells to the heart at baseline or after MI has not been investigated.

Methods

Here we generated Ly6a gene-targeted mice containing either a constitutive or an inducible Cre recombinase to perform genetic lineage tracing of Sca-1⁺ cells in vivo.

Results

We observed that the c

SUBMITTER: Vagnozzi RJ 

PROVIDER: S-EPMC6843990 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature