Longitudinal immune characterization of syngeneic tumor models to enable model selection for immune oncology drug discovery.

ABSTRACT: BACKGROUND:The ability to modulate immune-inhibitory pathways using checkpoint blockade antibodies such as αPD-1, αPD-L1, and αCTLA-4 represents a significant breakthrough in cancer therapy in recent years. This has driven interest in identifying small-molecule-immunotherapy combinations to increase the proportion of responses. Murine syngeneic models, which have a functional immune system, represent an essential tool for pre-clinical evaluation of new immunotherapies. However, immune response varies widely between models and the translational relevance of each model is not fully understood, making selection of an appropriate pre-clinical model for drug target validation challenging. METHODS:Using flow cytometry, O-link protein analysis, RT-PCR, and RNAseq we have characterized kinetic cha

SUBMITTER: Taylor MA 

PROVIDER: S-EPMC6883640 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature