The lytic activity of VSV-GP treatment dominates the therapeutic effects in a syngeneic model of lung cancer.

ABSTRACT:

Background

Oncolytic virotherapy is thought to result in direct virus-induced lytic tumour killing and simultaneous activation of innate and tumour-specific adaptive immune responses. Using a chimeric vesicular stomatitis virus variant VSV-GP, we addressed the direct oncolytic effects and the role of anti-tumour immune induction in the syngeneic mouse lung cancer model LLC1.

Methods

To study a tumour system with limited antiviral effects, we generated interferon receptor-deficient cells (LLC1-IFNAR1^-/-). Therapeutic efficacy of VSV-GP was assessed in vivo in syngeneic C57BL/6 and athymic nude mice bearing subcutaneous tumours. VSV-GP treatment effects were analysed using bioluminescent imaging (BLI), immunohistochemistry, ELISpot, flow cytometry, multiplex ELISA a

SUBMITTER: Schreiber LM 

PROVIDER: S-EPMC6889376 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature