Diagnosis of Vespa affinis venom allergy: use of immunochemical methods and a passive basophil activation test.

ABSTRACT:

Background

Allergy to Vespa affinis venom is common in the Asia Pacific region. Venom preparations for diagnosis are not commercially available for this species.

Methods

The prominent allergens in V. affinis venom were identifiedusing immunochemical methods. Use of ImmunoCAP of Vespula vulgaris crude venom/its components and a passive basophil activation test (BAT) in the diagnosis of patients who had anaphylaxis to V. affinis venom (n = 30) were also accessed. The IgE double-positivity rates (positive to both hornet and honeybee) in ImmunoCAP and the passive BAT were determined.

Results

High IgE reactivity was seen with the five allergens in V. affinis venom; 96% (29/30) for 34 and 24 kDa, 93% (28/30) for 45 kDa and 90% (27/30) reac

SUBMITTER: Gunasekara P 

PROVIDER: S-EPMC6894289 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature