The impact of storage conditions on human stool 16S rRNA microbiome composition and diversity.

ABSTRACT:

Background

Multiple factors can influence stool sample integrity upon sample collection. Preservation of faecal samples for microbiome studies is therefore an important step, particularly in tropical regions where resources are limited and high temperatures may significantly influence microbiota profiles. Freezing is the accepted standard to preserve faecal samples however, cold chain methods are often unfeasible in fieldwork scenarios particularly in low and middle-income countries and alternatives are required. This study therefore aimed to address the impact of different preservative methods, time-to-freezing at ambient tropical temperatures, and stool heterogeneity on stool microbiome diversity and composition under real-life physical environments found in resource-limited fiel

SUBMITTER: Carruthers LV 

PROVIDER: S-EPMC6894433 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature