Effector gene vap1 based DGGE fingerprinting to assess variation within and among Heterodera schachtii populations.

ABSTRACT: Populations of beet cyst nematodes Heterodera schachtii vary in aggressiveness and virulence toward sugar beet varieties, but also in traits like host range, or decline rate in the field. Diversity of their essential pathogenicity gene vap1 is shaped by diversifying selection and gene flow. The authors developed a technique to study inter-population variation and intra-population diversity and dynamics of H. schachtii based on the gene vap1. Degenerate primers were designed to amplify, clone, and sequence this gene from diverse species and populations of cyst nematodes. This resulted in a high diversity of sequences for H. schachtii, and allowed to design non-degenerated primers to amplify a fragment suitable for sequence dependent separation of gene vari

SUBMITTER: Nuaima RH 

PROVIDER: S-EPMC6909312 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature