Bone marrow-derived neural crest precursors improve nerve defect repair partially through secreted trophic factors.

ABSTRACT:

Background

Emerging evidence suggests that neural crest-derived cells (NCCs) present important functions in peripheral nerve regeneration to correct the insufficiency of autogenous Schwann cells. Postmigratory NCCs have been successfully isolated from adult rat bone marrow in our previous work. In this study, we aim to provide neural crest-derived Schwann cell precursors (SCPs) for repair of nerve defects in adult rats, and partially reveal the mechanisms involved in neuroregeneration of cell therapy.

Methods

A clonal cell line of neural crest precursors of rat bone marrow origin (rBM-NCPs) with SCP identity was expanded in adherent monolayer culture to ensure the stable cell viability of NCPs and potentiate the repair of nerve defects after rBM-NCPs implantation based on ti

SUBMITTER: Shi H 

PROVIDER: S-EPMC6921427 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature