Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene.

ABSTRACT:

Background

Yarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered Y. lipolytica is still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential of Y. lipolytica for terpenoid production.

Results

We constructed libraries of strains overexpressing mevalonate pathway and α-farnesene synthase genes by non-homologous end-joining (NHEJ) mediated integration into the Y. lipolytica chromosome. First, a mevalonate overproduction strain was selected by overexpressing relevant genes and changing the cof

SUBMITTER: Liu Y 

PROVIDER: S-EPMC6927232 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature