Dataset Information

Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene.

ABSTRACT:

Background

Yarrowia lipolytica, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered Y. lipolytica is still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential of Y. lipolytica for terpenoid production.

Results

We constructed libraries of strains overexpressing mevalonate pathway and α-farnesene synthase genes by non-homologous end-joining (NHEJ) mediated integration into the Y. lipolytica chromosome. First, a mevalonate overproduction strain was selected by overexpressing relevant genes and changing the cofactor specificity. Based on this strain, the downstream α-farnesene synthesis pathway was overexpressed by iterative integration. Culture conditions were also optimized. A strain that produced 25.55 g/L α-farnesene was obtained. This is the highest terpenoid titer reported in Y. lipolytica.

Conclusions

Yarrowia lipolytica is a potentially valuable species for terpenoid production, and NHEJ-mediated modular integration is effective for expression library construction and screening of high-producer strains.

SUBMITTER: Liu Y

PROVIDER: S-EPMC6927232 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature

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Publications

Engineering the oleaginous yeast <i>Yarrowia lipolytica</i> for production of α-farnesene.

Liu Yinghang Y Jiang Xin X Cui Zhiyong Z Wang Zhaoxuan Z Qi Qingsheng Q Hou Jin J

Biotechnology for biofuels 20191223

<h4>Background</h4><i>Yarrowia lipolytica</i>, a non-traditional oil yeast, has been widely used as a platform for lipid production. However, the production of other chemicals such as terpenoids in engineered <i>Y. lipolytica</i> is still low. α-Farnesene, a sesquiterpene, can be used in medicine, bioenergy and other fields, and has very high economic value. Here, we used α-farnesene as an example to explore the potential of <i>Y. lipolytica</i> for terpenoid production.<h4>Results</h4>We constr ...[more]

PMID: 31890024

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