Proteomics analysis of colon cancer progression.

ABSTRACT:

Background

The aim of this pilot study was to identify proteins associated with advancement of colon cancer (CC).

Methods

A quantitative proteomics approach was used to determine the global changes in the proteome of primary colon cancer from patients with non-cancer normal colon (NC), non-adenomatous colon polyp (NAP), non-metastatic tumor (CC NM) and metastatic tumor (CC M) tissues, to identify up- and down-regulated proteins. Total protein was extracted from each biopsy, trypsin-digested, iTRAQ-labeled and the resulting peptides separated using strong cation exchange (SCX) and reverse-phase (RP) chromatography on-line to electrospray ionization mass spectrometry (ESI-MS).

Results

Database searching of the MS/MS data resulted in the identification of 2777 proteins w

SUBMITTER: Saleem S 

PROVIDER: S-EPMC6935225 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature