Improving heterologous protein expression in Synechocystis sp. PCC 6803 for alpha-bisabolene production.

ABSTRACT: Cyanobacterial biofuels have the potential to reduce the cost and climate impacts of biofuel production because primary carbon fixation and conversion to fuel are completed together in the cultivation of the cyanobacteria. Cyanobacterial biofuels, therefore, do not rely on costly organic carbon feedstocks that heterotrophs require, which reduces competition for agricultural resources such as arable land and freshwater. However, the published product titer achieved for most molecules of interest using cyanobacteria lag behind what has been achieved using yeast and Escherichia coli (E. coli) cultures. In Synechocystis sp. PCC 6803 (S. 6803), we attempted to increase the product titer of the sesquiterpene, bisabolene, which may be converted to bisabolane, a possibl

SUBMITTER: Sebesta J 

PROVIDER: S-EPMC6940699 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature