SPRI Beads-based Size Selection in the Range of 2-10kb.
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ABSTRACT:
SUBMITTER: Stortchevoi A
PROVIDER: S-EPMC6944320 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Publications
SPRI Beads-based Size Selection in the Range of 2-10kb.
Stortchevoi Alexei A Kamelamela Noelani N Levine Stuart S SS
Journal of biomolecular techniques : JBT 20200401 1
Application of solid-phase reversible immobilization (SPRI) beads for size selection in molecular biology should be expanded, in light of the property of the beads to accommodate to high MW intervals of DNA fragment size selection, depending on composition of bead-suspension buffer. Here we show how the conventional size selection interval of 150-800 bp be shifted to 1.5-7 Kbp with by adjusting the concentration of NaCl in the stock suspension buffer. The MW capacity of SPRI beads also change wh ...[more]
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