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The specific ex vivo released cytokine profile is associated with ischemic stroke outcome and improves its prediction.

ABSTRACT: BACKGROUND:Inflammation is associated with poor outcome after stroke. A relationship between ex vivo cytokine synthesis and stroke outcome remains unclear. We explored an association between ex vivo cytokine release, circulating interleukin (IL)-6 as a marker of systemic inflammation, and stroke prognosis. We assessed the utility of ex vivo synthesized cytokines for predicting stroke outcome. METHODS:We collected blood from 248 ischemic stroke patients and stimulated it ex vivo with lipopolysaccharide. We measured concentration of synthesized cytokines (TNFα, IP-10, IL-1β, IL-6, IL-8, IL-10, and IL-12) and plasma IL-6. We assessed functional outcome 3 months after stroke using the modified Rankin Scale. To assess the prognostic ability of cytokines, we applied multivariate logistic regression, cluster analysis, and construction of multimarker score. RESULTS:Decreased release of IP-10, TNFα, IL-1β, and IL-12; increased release of IL-10 and IL-8; and higher plasma IL-6 level were associated with poor outcome. Cluster analysis identified three groups of patients with distinct cytokine profiles. The group with the worst outcome demonstrated high synthesis of IL-10, IL-8, IL-1β, and IL-6 and low synthesis of IL-12, IP-10, and TNFα accompanied by high circulating IL-6 level. The group with the best prognosis showed high synthesis of TNFα, IP-10, IL-12, IL-1β, and IL-6; low synthesis of IL-10 and IL-8; and low plasma IL-6. Patients with intermediate outcome had low synthesis of all cytokines accompanied by low circulating IL-6. We constructed a multimarker score composed of ex vivo released IL-12, IL-10, TNFα, and plasma IL-6. Addition of this score to clinical variables led to significant increase in c-statistic (0.81 vs 0.73, p = 0.02) and net reclassification improvement. CONCLUSION:The decreased ex vivo release of pro-inflammatory cytokines and increased release of IL-10 and IL-8 are related to poor outcome after stroke. Cytokine-based multimarker score adds prognostic value to clinical model for predicting stroke outcome.

SUBMITTER: Klimiec-Moskal E

PROVIDER: S-EPMC6945431 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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The specific ex vivo released cytokine profile is associated with ischemic stroke outcome and improves its prediction.

Klimiec-Moskal Elzbieta E Piechota Marcin M Pera Joanna J Weglarczyk Kazimierz K Slowik Agnieszka A Siedlar Maciej M Dziedzic Tomasz T

Journal of neuroinflammation 20200106 1

<h4>Background</h4>Inflammation is associated with poor outcome after stroke. A relationship between ex vivo cytokine synthesis and stroke outcome remains unclear. We explored an association between ex vivo cytokine release, circulating interleukin (IL)-6 as a marker of systemic inflammation, and stroke prognosis. We assessed the utility of ex vivo synthesized cytokines for predicting stroke outcome.<h4>Methods</h4>We collected blood from 248 ischemic stroke patients and stimulated it ex vivo wi ...[more]

PMID: 31906994

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The specific ex vivo released cytokine profile is associated with ischemic stroke outcome and improves its prediction.

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The specific ex vivo released cytokine profile is associated with ischemic stroke outcome and improves its prediction.

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