Characterization of the ferret TRB locus guided by V, D, J, and C gene expression analysis.
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ABSTRACT:
SUBMITTER: Gerritsen B
PROVIDER: S-EPMC6971162 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Publications
Characterization of the ferret TRB locus guided by V, D, J, and C gene expression analysis.
Gerritsen Bram B Pandit Aridaman A Zaaraoui-Boutahar Fatiha F van den Hout Mirjam C G N MCGN van IJcken Wilfred F J WFJ de Boer Rob J RJ Andeweg Arno C AC
Immunogenetics 20191203 1-2
The domestic ferret, Mustela putorius furo, is an important mammalian animal model to study human respiratory infection. However, insufficient genomic annotation hampers detailed studies of ferret T cell responses. In this study, we analyzed the published T cell receptor beta (TRB) locus and performed high-throughput sequencing (HTS) of peripheral blood of four healthy adult ferrets to identify expressed V, D, J, and C genes. The HTS data is used as a guide to manually curate the expressed V, D, ...[more]
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