Monitoring spatiotemporal changes in chaperone-mediated autophagy in vivo.

ABSTRACT: Autophagy malfunctioning occurs in multiple human disorders, making attractive the idea of chemically modulating it with therapeutic purposes. However, for many types of autophagy, a clear understanding of tissue-specific differences in their activity and regulation is missing because of lack of methods to monitor these processes in vivo. Chaperone-mediated autophagy (CMA) is a selective type of autophagy that until now has only been studied in vitro and not in the tissue context at single cell resolution. Here, we develop a transgenic reporter mouse that allows dynamic measurement of CMA activity in vivo using image-based procedures. We identify previously unknown spatial and temporal differences in CMA activity in multiple organs and in response to stress. We illustrate the versatility o

SUBMITTER: Dong S 

PROVIDER: S-EPMC6994528 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature