Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil.

ABSTRACT: BACKGROUND:Bacteriophages (phages) are the most numerous biological entities on Earth and play a crucial role in shaping microbial communities. Investigating the bacteriophage community from soil will shed light not only on the yet largely unknown phage diversity, but may also result in novel insights towards their functioning in the global biogeochemical nutrient cycle and their significance in earthbound ecosystems. Unfortunately, information about soil viromes is rather scarce compared to aquatic environments, due to the heterogeneous soil matrix, which rises major technical difficulties in the extraction process. Resolving these technical challenges and establishing a standardized extraction protocol is, therefore, a fundamental prerequisite for replicable results and comparative virom

SUBMITTER: Goller PC 

PROVIDER: S-EPMC7014677 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature