Estimating testosterone concentrations in adolescent girls: Comparison of two direct immunoassays to liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Context

The appropriate role of direct total testosterone (T) immunoassays in reproductive research is controversial.

Objective

To assess the concordance between two direct immunoassays and a liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for total T in adolescent girls with measured concentrations < 50 ng/dl.

Design

Cross-sectional analysis.

Setting

Academic medical center.

Participants

Adolescent girls (age 8.4-18.1 years) participating in clinical research protocols.

Intervention

Paired blood samples were obtained for total T by LC-MS/MS (n = 66; Mayo Clinic Laboratory) and by direct immunoassay (Center for Research in Reproduction)-either radioimmunoassay (RIA; n = 31) or chemiluminescence immunoassay (CLIA; n = 35). At the ti

SUBMITTER: McCartney CR 

PROVIDER: S-EPMC7017602 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature