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An Ustilago maydis chassis for itaconic acid production without by-products.


ABSTRACT: Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild-type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by-product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate biosynthesis pathway. With this strategy, a marker-free chassis strain could be engineered, which produces itaconate from glucose with significantly enhanced titre, rate and yield. The lack of by-product formation not only benefited itaconate production, it also increases the efficiency of downstream processing improving cell handling and product purity.

SUBMITTER: Becker J 

PROVIDER: S-EPMC7017832 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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An Ustilago maydis chassis for itaconic acid production without by-products.

Becker Johanna J   Hosseinpour Tehrani Hamed H   Gauert Marc M   Mampel Jörg J   Blank Lars M LM   Wierckx Nick N  

Microbial biotechnology 20191227 2


Ustilago maydis is a promising yeast for the production of a range of valuable metabolites, including itaconate, malate, glycolipids and triacylglycerols. However, wild-type strains generally produce a potpourri of all of these metabolites, which hinders efficient production of single target chemicals. In this study, the diverse by-product spectrum of U. maydis was reduced through strain engineering using CRISPR/Cas9 and FLP/FRT, greatly increasing the metabolic flux into the targeted itaconate  ...[more]

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