Precise and Programmable Detection of Mutations Using Ultraspecific Riboregulators.

ABSTRACT: The ability to identify single-nucleotide mutations is critical for probing cell biology and for precise detection of disease. However, the small differences in hybridization energy provided by single-base changes makes identification of these mutations challenging in living cells and complex reaction environments. Here, we report a class of de novo-designed prokaryotic riboregulators that provide ultraspecific RNA detection capabilities in vivo and in cell-free transcription-translation reactions. These single-nucleotide-specific programmable riboregulators (SNIPRs) provide over 100-fold differences in gene expression in response to target RNAs differing by a single nucleotide in E. coli and resolve single epitranscriptomic marks in vitro. By exploiting the programmable SNIPR design, we i

SUBMITTER: Hong F 

PROVIDER: S-EPMC7063572 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature