Autophagy-dependent filopodial kinetics restrict synaptic partner choice during Drosophila brain wiring.

ABSTRACT: Brain wiring is remarkably precise, yet most neurons readily form synapses with incorrect partners when given the opportunity. Dynamic axon-dendritic positioning can restrict synaptogenic encounters, but the spatiotemporal interaction kinetics and their regulation remain essentially unknown inside developing brains. Here we show that the kinetics of axonal filopodia restrict synapse formation and partner choice for neurons that are not otherwise prevented from making incorrect synapses. Using 4D imaging in developing Drosophila brains, we show that filopodial kinetics are regulated by autophagy, a prevalent degradation mechanism whose role in brain development remains poorly understood. With surprising specificity, autophagosomes form in synaptogenic filopodia, followed by filopodial colla

SUBMITTER: Kiral FR 

PROVIDER: S-EPMC7067798 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature