Proteomic identification of allergenic proteins in red oak (Quercus rubra) pollen.

ABSTRACT:

Background

Red oak pollen is an important cause of allergic respiratory disease and it is widely distributed in North America and central Europe. To date, however, red oak pollen allergens have not been identified. Here, we describe the allergenic protein profile from red oak pollen.

Methods

Total proteins were extracted from red oak pollen using a modified phenolic extraction method, and, subsequently, proteins were separated by two-dimensional gel electrophoresis (2DE) for both total protein stain (Coomassie Blue) and immunoblotting. A pool of 8 sera from red oak sensitive patients was used to analyze blotted proteins. Protein spots were analyzed by Mass Spectrometry.

Results

Electrophoretic pattern of total soluble proteins showed higher intensity bands in the regi

SUBMITTER: Huerta-Ocampo JA 

PROVIDER: S-EPMC7082215 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature